Light microscopes and fluorescent microscopes are the two types of equipment provided by binocular microscope manufacturers in India. These microscopes are used to detect the living cell’s cellular components and cells components.
Though they may look similar in function, these two kinds of microscopes are different. A fluorescent microscope is a special form of light microscope that can produce a better result and make microorganisms visible. However, there are so many differences between these microscopes, which are as follows:
What Is Light Microscope?
A light microscopy is a form of microscope which uses light and binocular magnifying lenses to see the tiny microorganisms. This is a common type of microscope in which most people use objects such as cells, microorganisms, and tissues.
Sometimes, dyes are also used on the cells and microbes to make the cells visible. Eosin and hematoxylin are two main forms of dyes used to stain the microbes and improve their visibility. Other forms of dyes used are crystal violet and safranin.
The microscope is quickly used to examine the cells and microbes. Usually, these microscopes are used in laboratories and hospitals to examine tissues, cells, and other microbes.
What is Fluorescence Microscopy?
Fluorescence microscopy is also a form of a light microscope but is a little advanced and has better tools for examining the images of the cells. In a fluorescence microscope in India, fluorescent dyes such as Fluorophores are applied to the sample by soaking the sample into the stain and injecting the dye.
When light from the microscope falls on the samples and strokes to the fluorophores, then longer-wavelength light enters the cells, and due to this, it reflects the light and creates an image.
The GFP (Green Fluorescent Protein) is helpful to emit the green and blue light of a longer wavelength. This type of microscope is used to check the cell structure and other features of the cells and microbes. The fluorescent microscope provides better vision and helps observe the genetic material too.
What Are the Key Differences Between the Light and Fluorescence Microscope in India?
The key differences between these microscopes are as follows:
- The light microscope uses 400-7700 nm wavelength light. At the same time, the Fluorescence microscope uses higher intensity light compared to the light microscope.
- The light microscope uses the light and dyes to make the microbes visible under a microscope. On the other hand, fluorescence microscopy uses fluorophores and light to better visualize the microbes under the light.
- Light microscopes are common and combine with dyes to make the cells, tissues visible. Moreover, these are easy to use whereas, a Fluorescence microscope is slightly difficult, and you need to inject fluorophores into the samples for better visibility.
- Light microscopes are used in common laboratories, but a fluorescence microscope is used in biopsy and genetic material diagnosing.
These are some of the key differences between light and fluorescence microscopes. However, light microscopy is used to examine the heat-fixed and live samples. On the other hand, fluorescence microscopy is used to observe the fixed samples.
For the best quality products, always ensure to buy a suitable microscope from Quasmo.