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microscope manufacturers in Ambala

Light microscopes and fluorescent microscopes are the two types of equipment provided by binocular microscope manufacturers in India. These microscopes are used to detect the living cell’s cellular components and cells components.

Though they may look similar in function, these two kinds of microscopes are different. A fluorescent microscope is a special form of light microscope that can produce a better result and make microorganisms visible. However, there are so many differences between these microscopes, which are as follows: 

What Is Light Microscope?

A light microscopy is a form of microscope which uses light and binocular magnifying lenses to see the tiny microorganisms. This is a common type of microscope in which most people use objects such as cells, microorganisms, and tissues. 

Sometimes, dyes are also used on the cells and microbes to make the cells visible. Eosin and hematoxylin are two main forms of dyes used to stain the microbes and improve their visibility. Other forms of dyes used are crystal violet and safranin. 

The microscope is quickly used to examine the cells and microbes. Usually, these microscopes are used in laboratories and hospitals to examine tissues, cells, and other microbes. 

What is Fluorescence Microscopy?

Fluorescence microscopy is also a form of a light microscope but is a little advanced and has better tools for examining the images of the cells. In a fluorescence microscope in India, fluorescent dyes such as Fluorophores are applied to the sample by soaking the sample into the stain and injecting the dye. 

When light from the microscope falls on the samples and strokes to the fluorophores, then longer-wavelength light enters the cells, and due to this, it reflects the light and creates an image. 

The GFP (Green Fluorescent Protein) is helpful to emit the green and blue light of a longer wavelength. This type of microscope is used to check the cell structure and other features of the cells and microbes. The fluorescent microscope provides better vision and helps observe the genetic material too. 

What Are the Key Differences Between the Light and Fluorescence Microscope in India? 

The key differences between these microscopes are as follows: 

  • The light microscope uses 400-7700 nm wavelength light. At the same time, the Fluorescence microscope uses higher intensity light compared to the light microscope. 
  • The light microscope uses the light and dyes to make the microbes visible under a microscope. On the other hand, fluorescence microscopy uses fluorophores and light to better visualize the microbes under the light.
  • Light microscopes are common and combine with dyes to make the cells, tissues visible. Moreover, these are easy to use whereas, a Fluorescence microscope is slightly difficult, and you need to inject fluorophores into the samples for better visibility. 
  • Light microscopes are used in common laboratories, but a fluorescence microscope is used in biopsy and genetic material diagnosing. 

Bottom Line 

These are some of the key differences between light and fluorescence microscopes. However, light microscopy is used to examine the heat-fixed and live samples. On the other hand, fluorescence microscopy is used to observe the fixed samples. 

For the best quality products, always ensure to buy a suitable microscope from Quasmo. 

 

Microscopes have been a major aspect of our science community for over centuries. Scientists and Biologists use microscopes to understand the nature of the universe and tiny living organisms and their functions in the sustainability of life. Over the years, the technology has gone a drastic change from simple dye-based photometry to imaging techniques. And this is why fluorescent microscopes have been invented.

India is a diverse land-based on geography and demography, and this technique can be used to determine the structure of different microorganisms dwelling with us. This eventually helps in understanding their uses and threats. Fluorescence technology has been around for a while, and many firms use it to generate images to a scale of 5 um. But we will not go much into the details. Instead, we will have a simple overview of the technique and understand why we need a fluorescent microscope in India.

Fluorescent microscope – a simple overview

A microscope is an instrument that magnifies tiny objects. Fluorescence microscopy is a technique through which the microscope detects a fluorescence signal, and an image is formed on the windows or monitor to show the labeled molecules. Fluorescence occurs when an electron moves from a lower energy state to a higher energy state. This emits light of a longer wavelength that is then captured by the fluorescent microscope. The detector then creates an image where you can see the samples in magnified form.

Indian context for fluorescent microscope

As already mentioned, India is a diverse country with a different demography. Scientists worldwide have been visiting India for research, and in the late 20th century, they introduced the concept of the fluorescent microscope in India. However, the equipment was highly sophisticated, and the cost of importing was plummeting towards the sky.

But the people in major research fields understood the requirement of such sophisticated equipment for their research. So, they found a way to bring the technology from the international borders to our land. They put their minds into understanding the engineering behind it and soon developed the prototype. This encouraged many people to do engineering on their part and make this equipment suited to the Indian market. Now, you can find many dealers and manufacturers for the fluorescent microscope in India and the products not burning a hole in your pocket. You can find some great microscope manufacturers in Ambala if you are looking forward to buying any microscope.

Applications for the fluorescent microscope in India

Now, we have come to the part where we will understand why we need the fluorescent microscope in India. There is a vast range of applications in the medical field for such instruments. People around the globe use this technique in detecting pathogens, cell functions, and Neuroscience.

The spectral vision imaging in the applications helps the researchers and specialist surgeons to find the root cause of the infectious diseases caused by bacteria, viruses, and other microorganisms. They can also detect the effect of certain chemical compounds on such microorganisms to test their medication effects. This will help in reducing the costs of vaccinations and medicines and effectively treat the disease.